Differential proteomic analysis of the secretome of Irpex lacteus and other white-rot fungi during wheat straw pretreatment.
Identifieur interne : 000377 ( Main/Exploration ); précédent : 000376; suivant : 000378Differential proteomic analysis of the secretome of Irpex lacteus and other white-rot fungi during wheat straw pretreatment.
Auteurs : Davinia Salvachúa [Espagne] ; Angel T. Martínez ; Ming Tien ; María F. L Pez-Lucendo ; Francisco García ; Vivian De Los Ríos ; María Jesús Martínez ; Alicia PrietoSource :
- Biotechnology for biofuels [ 1754-6834 ] ; 2013.
Abstract
BACKGROUND
Identifying new high-performance enzymes or enzyme complexes to enhance biomass degradation is the key for the development of cost-effective processes for ethanol production. Irpex lacteus is an efficient microorganism for wheat straw pretreatment, yielding easily hydrolysable products with high sugar content. Thus, this fungus was selected to investigate the enzymatic system involved in lignocellulose decay, and its secretome was compared to those from Phanerochaete chrysosporium and Pleurotus ostreatus which produced different degradation patterns when growing on wheat straw. Extracellular enzymes were analyzed through 2D-PAGE, nanoLC/MS-MS, and homology searches against public databases.
RESULTS
In wheat straw, I. lacteus secreted proteases, dye-decolorizing and manganese-oxidizing peroxidases, and H2O2 producing-enzymes but also a battery of cellulases and xylanases, excluding those implicated in cellulose and hemicellulose degradation to their monosaccharides, making these sugars poorly available for fungal consumption. In contrast, a significant increase of β-glucosidase production was observed when I. lacteus grew in liquid cultures. P. chrysosporium secreted more enzymes implicated in the total hydrolysis of the polysaccharides and P. ostreatus produced, in proportion, more oxidoreductases.
CONCLUSION
The protein pattern secreted during I. lacteus growth in wheat straw plus the differences observed among the different secretomes, justify the fitness of I. lacteus for biopretreatment processes in 2G-ethanol production. Furthermore, all these data give insight into the biological degradation of lignocellulose and suggest new enzyme mixtures interesting for its efficient hydrolysis.
DOI: 10.1186/1754-6834-6-115
PubMed: 23937687
PubMed Central: PMC3750859
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Differential proteomic analysis of the secretome of Irpex lacteus and other white-rot fungi during wheat straw pretreatment.</title><author><name sortKey="Salvachua, Davinia" sort="Salvachua, Davinia" uniqKey="Salvachua D" first="Davinia" last="Salvachúa">Davinia Salvachúa</name><affiliation wicri:level="2"><nlm:affiliation>Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, E-28040 Madrid, Spain. mjmartinez@cib.csic.es.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, E-28040 Madrid</wicri:regionArea><placeName><region nuts="2" type="communauté">Communauté de Madrid</region></placeName></affiliation></author><author><name sortKey="Martinez, Angel T" sort="Martinez, Angel T" uniqKey="Martinez A" first="Angel T" last="Martínez">Angel T. Martínez</name></author><author><name sortKey="Tien, Ming" sort="Tien, Ming" uniqKey="Tien M" first="Ming" last="Tien">Ming Tien</name></author><author><name sortKey="L Pez Lucendo, Maria F" sort="L Pez Lucendo, Maria F" uniqKey="L Pez Lucendo M" first="María F" last="L Pez-Lucendo">María F. L Pez-Lucendo</name></author><author><name sortKey="Garcia, Francisco" sort="Garcia, Francisco" uniqKey="Garcia F" first="Francisco" last="García">Francisco García</name></author><author><name sortKey="De Los Rios, Vivian" sort="De Los Rios, Vivian" uniqKey="De Los Rios V" first="Vivian" last="De Los Ríos">Vivian De Los Ríos</name></author><author><name sortKey="Martinez, Maria Jesus" sort="Martinez, Maria Jesus" uniqKey="Martinez M" first="María Jesús" last="Martínez">María Jesús Martínez</name></author><author><name sortKey="Prieto, Alicia" sort="Prieto, Alicia" uniqKey="Prieto A" first="Alicia" last="Prieto">Alicia Prieto</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2013">2013</date><idno type="RBID">pubmed:23937687</idno><idno type="pmid">23937687</idno><idno type="doi">10.1186/1754-6834-6-115</idno><idno type="pmc">PMC3750859</idno><idno type="wicri:Area/Main/Corpus">000356</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000356</idno><idno type="wicri:Area/Main/Curation">000356</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000356</idno><idno type="wicri:Area/Main/Exploration">000356</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Differential proteomic analysis of the secretome of Irpex lacteus and other white-rot fungi during wheat straw pretreatment.</title><author><name sortKey="Salvachua, Davinia" sort="Salvachua, Davinia" uniqKey="Salvachua D" first="Davinia" last="Salvachúa">Davinia Salvachúa</name><affiliation wicri:level="2"><nlm:affiliation>Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, E-28040 Madrid, Spain. mjmartinez@cib.csic.es.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, E-28040 Madrid</wicri:regionArea><placeName><region nuts="2" type="communauté">Communauté de Madrid</region></placeName></affiliation></author><author><name sortKey="Martinez, Angel T" sort="Martinez, Angel T" uniqKey="Martinez A" first="Angel T" last="Martínez">Angel T. Martínez</name></author><author><name sortKey="Tien, Ming" sort="Tien, Ming" uniqKey="Tien M" first="Ming" last="Tien">Ming Tien</name></author><author><name sortKey="L Pez Lucendo, Maria F" sort="L Pez Lucendo, Maria F" uniqKey="L Pez Lucendo M" first="María F" last="L Pez-Lucendo">María F. L Pez-Lucendo</name></author><author><name sortKey="Garcia, Francisco" sort="Garcia, Francisco" uniqKey="Garcia F" first="Francisco" last="García">Francisco García</name></author><author><name sortKey="De Los Rios, Vivian" sort="De Los Rios, Vivian" uniqKey="De Los Rios V" first="Vivian" last="De Los Ríos">Vivian De Los Ríos</name></author><author><name sortKey="Martinez, Maria Jesus" sort="Martinez, Maria Jesus" uniqKey="Martinez M" first="María Jesús" last="Martínez">María Jesús Martínez</name></author><author><name sortKey="Prieto, Alicia" sort="Prieto, Alicia" uniqKey="Prieto A" first="Alicia" last="Prieto">Alicia Prieto</name></author></analytic><series><title level="j">Biotechnology for biofuels</title><idno type="ISSN">1754-6834</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b></p><p>Identifying new high-performance enzymes or enzyme complexes to enhance biomass degradation is the key for the development of cost-effective processes for ethanol production. Irpex lacteus is an efficient microorganism for wheat straw pretreatment, yielding easily hydrolysable products with high sugar content. Thus, this fungus was selected to investigate the enzymatic system involved in lignocellulose decay, and its secretome was compared to those from Phanerochaete chrysosporium and Pleurotus ostreatus which produced different degradation patterns when growing on wheat straw. Extracellular enzymes were analyzed through 2D-PAGE, nanoLC/MS-MS, and homology searches against public databases.</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>In wheat straw, I. lacteus secreted proteases, dye-decolorizing and manganese-oxidizing peroxidases, and H2O2 producing-enzymes but also a battery of cellulases and xylanases, excluding those implicated in cellulose and hemicellulose degradation to their monosaccharides, making these sugars poorly available for fungal consumption. In contrast, a significant increase of β-glucosidase production was observed when I. lacteus grew in liquid cultures. P. chrysosporium secreted more enzymes implicated in the total hydrolysis of the polysaccharides and P. ostreatus produced, in proportion, more oxidoreductases.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSION</b></p><p>The protein pattern secreted during I. lacteus growth in wheat straw plus the differences observed among the different secretomes, justify the fitness of I. lacteus for biopretreatment processes in 2G-ethanol production. Furthermore, all these data give insight into the biological degradation of lignocellulose and suggest new enzyme mixtures interesting for its efficient hydrolysis.</p></div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">23937687</PMID><DateCompleted><Year>2013</Year><Month>08</Month><Day>15</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Print">1754-6834</ISSN><JournalIssue CitedMedium="Print"><Volume>6</Volume><Issue>1</Issue><PubDate><Year>2013</Year><Month>Aug</Month><Day>10</Day></PubDate></JournalIssue><Title>Biotechnology for biofuels</Title><ISOAbbreviation>Biotechnol Biofuels</ISOAbbreviation></Journal><ArticleTitle>Differential proteomic analysis of the secretome of Irpex lacteus and other white-rot fungi during wheat straw pretreatment.</ArticleTitle><Pagination><MedlinePgn>115</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1186/1754-6834-6-115</ELocationID><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">Identifying new high-performance enzymes or enzyme complexes to enhance biomass degradation is the key for the development of cost-effective processes for ethanol production. Irpex lacteus is an efficient microorganism for wheat straw pretreatment, yielding easily hydrolysable products with high sugar content. Thus, this fungus was selected to investigate the enzymatic system involved in lignocellulose decay, and its secretome was compared to those from Phanerochaete chrysosporium and Pleurotus ostreatus which produced different degradation patterns when growing on wheat straw. Extracellular enzymes were analyzed through 2D-PAGE, nanoLC/MS-MS, and homology searches against public databases.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">In wheat straw, I. lacteus secreted proteases, dye-decolorizing and manganese-oxidizing peroxidases, and H2O2 producing-enzymes but also a battery of cellulases and xylanases, excluding those implicated in cellulose and hemicellulose degradation to their monosaccharides, making these sugars poorly available for fungal consumption. In contrast, a significant increase of β-glucosidase production was observed when I. lacteus grew in liquid cultures. P. chrysosporium secreted more enzymes implicated in the total hydrolysis of the polysaccharides and P. ostreatus produced, in proportion, more oxidoreductases.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">The protein pattern secreted during I. lacteus growth in wheat straw plus the differences observed among the different secretomes, justify the fitness of I. lacteus for biopretreatment processes in 2G-ethanol production. Furthermore, all these data give insight into the biological degradation of lignocellulose and suggest new enzyme mixtures interesting for its efficient hydrolysis.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Salvachúa</LastName><ForeName>Davinia</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, E-28040 Madrid, Spain. mjmartinez@cib.csic.es.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Martínez</LastName><ForeName>Angel T</ForeName><Initials>AT</Initials></Author><Author ValidYN="Y"><LastName>Tien</LastName><ForeName>Ming</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>López-Lucendo</LastName><ForeName>María F</ForeName><Initials>MF</Initials></Author><Author ValidYN="Y"><LastName>García</LastName><ForeName>Francisco</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>de Los Ríos</LastName><ForeName>Vivian</ForeName><Initials>V</Initials></Author><Author ValidYN="Y"><LastName>Martínez</LastName><ForeName>María Jesús</ForeName><Initials>MJ</Initials></Author><Author ValidYN="Y"><LastName>Prieto</LastName><ForeName>Alicia</ForeName><Initials>A</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2013</Year><Month>08</Month><Day>10</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Biotechnol Biofuels</MedlineTA><NlmUniqueID>101316935</NlmUniqueID><ISSNLinking>1754-6834</ISSNLinking></MedlineJournalInfo></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2013</Year><Month>06</Month><Day>07</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2013</Year><Month>08</Month><Day>06</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>8</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>8</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2013</Year><Month>8</Month><Day>14</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">23937687</ArticleId><ArticleId IdType="pii">1754-6834-6-115</ArticleId><ArticleId IdType="doi">10.1186/1754-6834-6-115</ArticleId><ArticleId IdType="pmc">PMC3750859</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Appl Microbiol Biotechnol. 2010 May;86(6):1903-14</Citation><ArticleIdList><ArticleId IdType="pubmed">20306191</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Genet. 2005 Jan;47(1):49-56</Citation><ArticleIdList><ArticleId IdType="pubmed">15551134</ArticleId></ArticleIdList></Reference><Reference><Citation>Proteomics. 2010 Aug;10(16):3020-34</Citation><ArticleIdList><ArticleId IdType="pubmed">20564262</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 2008 Aug;74(16):5106-12</Citation><ArticleIdList><ArticleId IdType="pubmed">18586974</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2012;7(12):e50571</Citation><ArticleIdList><ArticleId IdType="pubmed">23227186</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Biotechnol. 2004 Jun;22(6):695-700</Citation><ArticleIdList><ArticleId IdType="pubmed">15122302</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioresour Technol. 2012 Jun;114:589-96</Citation><ArticleIdList><ArticleId IdType="pubmed">22459963</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 1999 Apr 9;274(15):10324-30</Citation><ArticleIdList><ArticleId IdType="pubmed">10187820</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 1990 May 5;265(13):7207-15</Citation><ArticleIdList><ArticleId IdType="pubmed">2158993</ArticleId></ArticleIdList></Reference><Reference><Citation>Proteomics. 2005 Dec;5(18):4832-8</Citation><ArticleIdList><ArticleId IdType="pubmed">16281184</ArticleId></ArticleIdList></Reference><Reference><Citation>Microbiology. 2007 Sep;153(Pt 9):3023-33</Citation><ArticleIdList><ArticleId IdType="pubmed">17768245</ArticleId></ArticleIdList></Reference><Reference><Citation>Int Microbiol. 2005 Sep;8(3):195-204</Citation><ArticleIdList><ArticleId IdType="pubmed">16200498</ArticleId></ArticleIdList></Reference><Reference><Citation>Fungal Genet Biol. 2006 May;43(5):343-56</Citation><ArticleIdList><ArticleId IdType="pubmed">16524749</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioresour Technol. 2013 May;135:89-92</Citation><ArticleIdList><ArticleId IdType="pubmed">23069603</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Microbiol Biotechnol. 2008 Sep;80(4):719-33</Citation><ArticleIdList><ArticleId IdType="pubmed">18654772</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 2013 Jul;79(14):4316-24</Citation><ArticleIdList><ArticleId IdType="pubmed">23666335</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Genomics. 2012 Feb 02;13:57</Citation><ArticleIdList><ArticleId IdType="pubmed">22300648</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioresour Technol. 2012 May;111:261-7</Citation><ArticleIdList><ArticleId IdType="pubmed">22406100</ArticleId></ArticleIdList></Reference><Reference><Citation>Folia Microbiol (Praha). 2009 Sep;54(5):375-90</Citation><ArticleIdList><ArticleId IdType="pubmed">19937209</ArticleId></ArticleIdList></Reference><Reference><Citation>Eur J Biochem. 2001 Sep;268(17):4787-93</Citation><ArticleIdList><ArticleId IdType="pubmed">11532015</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2011 May 20;286(20):17560-8</Citation><ArticleIdList><ArticleId IdType="pubmed">21454637</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biotechnol. 1997 Jul 23;56(1):1-24</Citation><ArticleIdList><ArticleId IdType="pubmed">9246788</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioresour Technol. 2011 Aug;102(16):7500-6</Citation><ArticleIdList><ArticleId IdType="pubmed">21646018</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2011 Apr 29;286(17):15586-96</Citation><ArticleIdList><ArticleId IdType="pubmed">21388958</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Pathol. 2012 Jun;13(5):445-53</Citation><ArticleIdList><ArticleId IdType="pubmed">22044785</ArticleId></ArticleIdList></Reference><Reference><Citation>Fungal Genet Biol. 2007 Feb;44(2):77-87</Citation><ArticleIdList><ArticleId IdType="pubmed">16971147</ArticleId></ArticleIdList></Reference><Reference><Citation>J Agric Food Chem. 2010 Oct 27;58(20):10893-8</Citation><ArticleIdList><ArticleId IdType="pubmed">20882980</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Microbiol Biotechnol. 2008 Sep;80(3):381-8</Citation><ArticleIdList><ArticleId IdType="pubmed">18636256</ArticleId></ArticleIdList></Reference><Reference><Citation>J Proteomics. 2012 Dec 21;77:298-309</Citation><ArticleIdList><ArticleId IdType="pubmed">23000217</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Microbiol Biotechnol. 2011 Sep;91(6):1477-92</Citation><ArticleIdList><ArticleId IdType="pubmed">21785931</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechnol Biofuels. 2011 Oct 11;4(1):37</Citation><ArticleIdList><ArticleId IdType="pubmed">21985037</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Microbiol Biotechnol. 2008 Jan;77(6):1241-50</Citation><ArticleIdList><ArticleId IdType="pubmed">18038130</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechnol Adv. 2009 Mar-Apr;27(2):185-94</Citation><ArticleIdList><ArticleId IdType="pubmed">19100826</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biotechnol. 2005 Jul 21;118(1):17-34</Citation><ArticleIdList><ArticleId IdType="pubmed">15888348</ArticleId></ArticleIdList></Reference><Reference><Citation>Anal Biochem. 1984 Apr;138(1):141-3</Citation><ArticleIdList><ArticleId IdType="pubmed">6731838</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochemistry. 2010 Apr 20;49(15):3305-16</Citation><ArticleIdList><ArticleId IdType="pubmed">20230050</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioresour Technol. 2013 Mar;131:218-25</Citation><ArticleIdList><ArticleId IdType="pubmed">23347930</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Espagne</li></country><region><li>Communauté de Madrid</li></region></list><tree><noCountry><name sortKey="De Los Rios, Vivian" sort="De Los Rios, Vivian" uniqKey="De Los Rios V" first="Vivian" last="De Los Ríos">Vivian De Los Ríos</name><name sortKey="Garcia, Francisco" sort="Garcia, Francisco" uniqKey="Garcia F" first="Francisco" last="García">Francisco García</name><name sortKey="L Pez Lucendo, Maria F" sort="L Pez Lucendo, Maria F" uniqKey="L Pez Lucendo M" first="María F" last="L Pez-Lucendo">María F. L Pez-Lucendo</name><name sortKey="Martinez, Angel T" sort="Martinez, Angel T" uniqKey="Martinez A" first="Angel T" last="Martínez">Angel T. Martínez</name><name sortKey="Martinez, Maria Jesus" sort="Martinez, Maria Jesus" uniqKey="Martinez M" first="María Jesús" last="Martínez">María Jesús Martínez</name><name sortKey="Prieto, Alicia" sort="Prieto, Alicia" uniqKey="Prieto A" first="Alicia" last="Prieto">Alicia Prieto</name><name sortKey="Tien, Ming" sort="Tien, Ming" uniqKey="Tien M" first="Ming" last="Tien">Ming Tien</name></noCountry><country name="Espagne"><region name="Communauté de Madrid"><name sortKey="Salvachua, Davinia" sort="Salvachua, Davinia" uniqKey="Salvachua D" first="Davinia" last="Salvachúa">Davinia Salvachúa</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PhanerochaeteV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000377 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000377 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PhanerochaeteV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:23937687
|texte= Differential proteomic analysis of the secretome of Irpex lacteus and other white-rot fungi during wheat straw pretreatment.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:23937687" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PhanerochaeteV1
| This area was generated with Dilib version V0.6.37. |  |